N1 Lab Solutions
RiboCraft Alpha
Revolutionizing Your RNA Research Toolbox
Who we are
“N1 Lab leverages core technologies originally developed at Stanford University, built upon decades of research in nucleic acid delivery. Dedicated to innovation and breakthroughs in RNA and DNA delivery technology, N1 Lab follows the rigorous pathways, strategies, and quality standards of drug development. With a focus on the develop ment of cutting-edge biomolecular delivery systems and optimized molecular formulations, N1 Lab applies these advanced technologies to gene delivery-achieving highly efficient transfection and stability both inside and outside cells. This enables groundbreaking solutions in gene delivery, gene therapy, and organ targeting.”
RiboCraft Alpha
>> Simple formulation protocol
>> Fast, controlled, and efficient expression
>> Buffer compatibility
>> Enhanced long-term stability
>> Stable particle size
>> Safe in vitro & in vivo
Product Features
Enhanced Efficiency
Our reagent significantly improves transfection efficiency, saving researchers time and resources
Stability and Consistency
With high stability, our reagent consistently delivers reliable results, ensuring reproducibility in experiments
Low Toxicity
User-Friendly
Our reagent has minimal cytotoxicity, reducing the risk of cell damage and influencing accurate data interpretation
Designed with ease of use in mind, our reagent simplifies the transfection process, enabling researchers to focus on their experiments
What is RiboCraft?
Ribo (Ribonucleic Acid)
RNA, a fundamental molecule in biology that plays a crucial role in the coding, decoding, regulation, and expression of genes.
Craft
An activity involving technique and expertise in making things perfect. When used in a technological or scientific context, it implies the skillful creation or engineering of something.
Introduction
RiboCraft Alpha, an RNA and DNA delivery tool independently developed by N1 Life, Inc., demonstrates gene expression and transfection efficiency across a wide range of cell lines. RiboCraft Alpha is physicochemically stable, water-soluble, and exhibits a strong safety profile. Its simple protocol allows for use under standard laboratory conditions, and the prepared formulation remains stable at room temperature for up to 30 minutes-ensuring reproducible and consistent results.
Experimental data demonstrate that RiboCraft Alpha is compatible with different types of physiological buffers and maintains consistent transfection efficiency under pH conditions ranging from 4 to 8. It is serum-tolerant during mRNA transfection and remains stable under varying temperature conditions. This versatility makes it suitable for use in diverse and complex biological environments. Moreover, by adjusting the ratio of RiboCraft Alpha to mRNA, high transfection efficiency can be achieved across a wide range of mRNA doses, enhancing its applicability in diverse delivery applications.
RiboCraft Alpha not only outperforms industry-standard reagents in mRNA transfection, but also delivers superior performance in siRNA applications. Its powerful performance extends to gene editing, achieving up to 95% in vitro editing efficiency with Cas9 mRNA and sgRNA co-delivery. Furthermore, it demonstrates comparable efficiency to PEI in plasmid DNA transfection for protein expression. RiboCraft Alpha is a versatile and robust delivery platform, designed to accelerate progress in gene therapy and molecular biotechnology.
RiboCraft Alpha
N1 Lab’s RiboCraft technology is evolved from the RNA and DNA delivery technology developed by N1 Life . It is designed and produced at the highest standards for efficacy, safety and stability.
90%-99%
mRNA transfection in immortalized cell lines
97%-99%
mRNA encapsulation efficiency
99%
>90% cell viability in most
tested cell lines
Versatile in Performance
In laboratory settings, RiboCraft Alpha demonstrates exceptional effectiveness in vitro, outperforming competitors in transfecting various cell cultures with mRNA, leading to remarkably high levels of gene expression and cellular uptake.
RiboCraft Alpha：No other product on the market achieves the same level of efficient and consistent mRNA delivery in animal models, marking RiboCraft Alpha as a groundbreaking leader in achieving enhanced gene expression with unmatched success.
RiboCraft Alpha is a robust tool to encapsulate, protect and deliver multi-component RNA mixtures. It has shown superior delivery capability of CRISPR/Cas gene editing system in cellular models when compared to marketed product.
Robust Application
RiboCraft Alpha sets itself apart as a one-of-a-kind transfection reagent, uniquely suited for both in vitro and in vivo RNA and plasmid DNA transfection. This dual-function capability is a rare find in the market, offering unparalleled convenience and efficiency for researchers exploring the frontiers of genetic manipulation. RiboCraft Alpha offers research programs the unique ability to directly translate from cell-based assays to animal studies.
MIX
Dilute nucleic acids and mix with RiboCraft reagent in aqueous buffer by gentle vortex or pipet mixing
TREAT
Incubate cells with nucleic acids/RiboCraft reagent under standard conditions for 4-72 hours
READ
Characterize and quantify protein expression by fluorescent methods or sequencing
